FrameStar 2-component技术减少热expansion and sample evaporation. The polycarbonate frame of FrameStar plates is significantly more heat resistant than standard PP plates, which reduces thermal expansion to a minimum. For this reason the seal integrity remains intact even at elevated temperatures during PCR.
To illustrate this advantage of our two-component technology we have compared evaporation from one piece PP plates and FrameStar PCR plates. Each well of a non-skirted 96 well plate (single piece, PP) and a Framestar non-skirted design (code 4ti-0710) was filled with 10 μl H2O. The plates were sealed with a qPCR adhesive (code 4ti-0560) and the weight of plates was measured before and after performing PCR (30 cycles x 15’’95°C; 15’’55°C).
Table 1shows that the average volume loss from one piece PP plates was 2.3μl per well which is equal to 23% of the total reaction volume. in contrast the volume loss from Framestar plates was only 0.49μl per well using adhesive sealing. For easier sample identification, we supply all microplate designs with a printed alphanumeric grid reference.
Plate type |
FrameStar plate (4ti-0710) |
1-component plate |
Starting weight |
26.678g |
17.807G |
Weight post PCR |
26.631g |
17.586g |
减肥 |
0.047g |
0.221g |
Volume loss total/well |
47µL/0.49µL |
221µl/2.3µl |
Table 1: Weight and volume loss from 96 well PCR plates. Results shown are averages from 5 plates of each plate type. One piece PP plates showed more than 4 times higher volume loss than FrameStar plates.